Cannabis is the most widely abused illicit drug in the US. Results from the 2013 National Survey on Drug Use and Health show that between 2007 and 2012 the rate of marijuana use rose from 5.8% to 7.3%. As use of marijuana increases, it becomes more prevalent in clinical and forensic case work, particularly in impaired driving cases. In 2007 the National Highway Traffic Safety Administration (NHTSA) reported that 8.6% of nighttime drivers tested positive for cannabinoids—a rate almost four times higher than those with blood alcohol levels equal to or above 0.8 g/L. Recent marijuana use is associated with a 2–6 fold increased crash risk, depending on dose, than when unimpaired.   The LC-MS/MS method developed in this study minimizes required sample volume and provides sensitive quantitation of THC, 11-OH-THC and THC-COOH in whol...

Automation Compatibility: Hamilton and Integra Oral fluid is gaining popularity as an alternative matrix for drug analysis. Oral fluid collection is simple, non-invasive, and can be easily monitored. Like other complex biological matrices, sample preparation is required to remove matrix interferents from oral fluid samples prior to LC-MS/MS analysis. This procedure can be time-consuming and is often the “bottle neck” for laboratory analysis and throughput. Dispersive Pipette XTRaction methods require much less solvent and time-consuming steps compared to other SPE techniques. Automation of the extraction is easy to achieve with XTR tips for Hamilton or Integra liquid handlers. Automated Dispersive Pipette XTRaction is highly reproducible and provides the sensitivity necessary for forensic and clinical purposes.   1 Condition Using Mix...

Cleanup Method for Rapid Analysis in Biological Samples Benzodiazepines and sedatives are some of the most commonly abused drugs in the nation and are often found in cases involving driving under the influence of drugs, sexual assaults and overdoses. Unfortunately, most existing methods used to identify benzodiazepines and sedatives across matrices are labor intensive and time-consuming. A simple cleanup method that can be applied across multiple biological matrices would be an ideal solution to increase the throughput capabilities of forensic laboratories. Unlike traditional methods for solid phase extraction, dispersive pipette extraction technology for clean-up focuses on removing matrix interferences, while keeping the benzodiazepines in the sample solution. XTR tips containing loose weak anion exchange (WAX) sorbent remove phospholipids and small non-amphoteric acids...

概述 阿密曲替林是一种广谱的三环抗抑郁药物，环苯扎林是一种三环骨骼肌松弛剂，其在代谢中均形成季铵葡萄糖苷酸复合物。为了能够精确的定量这些代谢物，需对复合物进行水解。本研究比较了四种β-葡萄糖醛酸酶对这两种复合物的水解效率。   材料与方法 1.      药物标准品购于Cerilliant Corporation。IMCSzyme®购于Integrated Micro-Chromatography Systems, LLC。鲍鱼β-葡萄糖醛酸酶购于Campbell Science，帽贝及大蜗牛β-葡萄糖醛酸酶购于Sigma-Aldrich。 2.      样本准备：200 μL尿液+100 μL水解复合物（水解缓冲液，酶及内标）。于60℃进行水解。IMCSzyme®水解时间为30分钟，其余三种酶水解60分钟。 3.      水解后经RP-S枪头（DPX LLC）萃取，并用LC-MS/MS分析。   结果 阿密曲替林与环苯扎林葡萄糖苷酸复合物能够被IMCSzyme®完全水解，其余三种酶只能部分水解，且...

Extraction < 15 minutesAutomation Compatibility: Hamilton The need for fast robust methods of evaluating cannabinoid content continues to rise with the increase in cannabis use. The chemical complexity of cannabis plant material and subsequent extracts may benefit from solid phase extraction prior to LC-MS/MS analysis. Current procedures either have very laborious sample preparation methods or lack them altogether. An analytical procedure for the determination of 9 cannabinoids in plant material was developed using Dispersive Pipette XTRaction on a Hamilton Microlab NIMBUS96 platform followed by LC-MS/MS analysis. Dispersive Pipette XTRaction requires minimal sorbent resulting in reduced matrix effects and reasonable levels of THC to avoid saturating the detector. The automated extraction method can process up to 96 samples simultaneously in u...