Automated Dispersive Pipette Extraction of Catecholamines and Metabolites from Urine Using a Nimbus96 and LC-MS/MS Extraction < 15 minutesAutomation Compatibility: Hamilton The catecholamines, epinephrine, norepinephrine, and dopamine, are bioamines that play an integral role as neurotransmitters in the central and peripheral nervous system. Screening for catecholamines and their O-methylated metabolites, metanephrine and normetanephrine, is a widely accepted approach for diagnosis of catecholamine-secreting tumors, such as pheochromocytomas, neuroblastomas, and paragangliomas. Catecholamines are characterized by a monoamine-linked benzene ring with two vicinyl hydroxyl groups (catechol). Epinephrine is a secondary amine, while norepinephrine and dopamine are primary amines. Under neutral and alkaline conditions, the catechol group makes the catecholamines vul...

Tip-on-Tip Urine Filtration Beta-glucuronidase enzyme removal, 96 samples in <5 minutesAutomation Compatibility: Hamilton Demonstrate the utility of DPX Low Porosity Filtration Tip for beta-glucuronidase enzyme and particulate removal prior to analysis Fast INTip Filtration method that will help extend LC column life and reduce instrument maintenance Improve robustness of dilute and shoot methods resulting in minimal loss of drug Process up to 96 samples in under 5 minutes replacing labor intensive filtration plates or centrifugation steps     Percent Recovery of Pain Management Drugs using ToT Urine Filtration Results for the recovery of drug compounds in 1:1 methanol:water All sample preparation for this method was performed on a Hamilton Nimbus96 shown here.   Low Porosity Filtration Tips <1 μm High efficiency sub-micr...

Automated Comprehensive Extraction of Drugs of Abuse in Urine Automation Compatibility: Hamilton, Tecan and Integra Clinical and forensic laboratories have historically used liquid or solid phase extraction (SPE) methods for analysis of drugs and metabolites in urine. SPE methods produce clean extracts with consistent, high quality data and ensure long term robustness of mass spectrometers. Yet the time-consuming nature and relatively high cost of SPE have pushed laboratories to implement alternative strategies. Approaches have focused on expensive LC-MS/MS instrumentation using a variety of “dilute and shoot” sample preparation methods to address “dirty” samples. While dilute and shoot methods are perceived as inexpensive, high-end ultra sensitive instrumentation and increased data analysis time are often required. Dilute and shoot ...