Desalting peptides is an essential procedure for improving LC-MS/MS analysis (1-3). Removing salts utilizes solid phase extraction
(SPE), which has relied on cartridge formats. Tese formats have been used for routine sample preparation and the microspin columnshave been developed to miniaturize and speed up the desalting processes. However, these procedures are not readily adapted forautomated liquid handlers. Here, we present automated, dispersive pipette extraction technology in IMCStips for robust and
high-throughput proteomics analysis. Several different resins were compared in spin column versus dispersive pipette (4). Specificpeptides were monitored for optimal loading and recoveries for both SPE formats (spin vs tip), and total proteome analysis wasassessed on cell lysates to determine total protein IDs for both extraction processes.