Developed and optimized a completely automated phosphopeptide enrichment method using two different resins in the IMCStip that allows for high-throughput and reproducible sample preparation.
• Identified of over 3000 phosphopeptides per sample with an average sample to sample peptide overlap of 80%.
• Generated reproducible quantitative MS data using a SILAC approach to compare treated versus untreated
phosphorylation profiles for the Hct116 cell line