JA0041 Development and validation of a streamlined method designed to detect residues of 62 veterinary drugs in bovine kidney using ultrahigh performance liquid chromatography – tandem mass spectrometry
来源:Drug Testing and Analysis
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作者:Steven J. Lehotay
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发布时间: 2066天前
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2566 次浏览
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In the USA, the US Department of Agriculture’s Food Safety and Inspection Service (FSIS) conducts the National Residue Program designed to monitor veterinary drug and other chemical residues in beef and other slaughtered food animals. Currently, FSIS uses a 7-plate bioassay in the laboratory to screen for antimicrobial drugs in bovine kidneys from those animals tested positive by inspectors in the slaughter establishments. The microbial inhibition bioassay has several limitations in terms of monitoring scope, sensitivity, selectivity, and analysis time. Ultra-high performance liquid chromatography – tandem mass spectrometry (UHPLC-MS/MS) has many advantages over the bioassay for this application, and this study was designed to develop, evaluate, and validate a fast UHPLC-MS/MS method for antibiotics and other high-priority veterinary drugs in bovine kidney. Five existing multi-class, multi-residue methods from the literature were tested and compared, and each performed similarly. Experiments with incurred samples demonstrated that a 5-min shake of 2 g homogenized kidney with 10 ml of 4/1 (v/v) acetonitrile/water followed by simultaneous clean-up of the initial extract with 0.5 g C18 and 10 ml hexane gave a fast, simple, and effective sample preparation method for the
1 Introduction
Analytical procedures typically consist of a number of equally important
steps for sampling, sample treatment, isolation of the target
Nearly all countries regulate the use of veterinary drugs for
food animal production to ensure that drug residues in the food (or target
tissue) do not exceed maximum residue levels (MRLs) or ‘tolerances’ in the USA.[1–5] Internationally,
the Codex Committee on Residues of Veterinary Drugs in Foods makes MRL and
other recommendations designed to provide international standards.[2] Monitoring of veterinary drug residues is conducted by many government
and contract laboratories around the world to enforce regulations domestically
and in international food trade. In the USA, the Department of Agriculture
(USDA) Food Safety and Inspection Service (FSIS) is responsible for the
scheduled surveillance and inspector-generated enforcement monitoring of
veterinary drugs and other adulterants in meat and poultry products.[3] The FSIS National Residue Program (NRP) has been in place since
1967 to conduct monitoring of imported and domestic meat, poultry, and egg
products collected from ports and processing establishments across the USA.[6] In the USA, all slaughter establishments that meet USDA
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