PO0015 The Importance of pH in β-Glucuronidase Hydrolysis Efficiency
来源:MicroSep
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作者:P. Nikki Sitasuwan*1,
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发布时间: 1714天前
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1972 次浏览
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•Different enzymes have different optimal hydrolysis pH
•pH optima are substrate dependent
•Urine samples have a range of pH 4.0-9.0 and require buffers to adjust pH for the most efficient hydrolysis
•Comparison of twoβ-glucuronidase hydrolysis efficiencies at their optimal pH in both fortified synthetic urine and authentic patient urine samples
•pH optima are substrate dependent
•Urine samples have a range of pH 4.0-9.0 and require buffers to adjust pH for the most efficient hydrolysis
•Comparison of twoβ-glucuronidase hydrolysis efficiencies at their optimal pH in both fortified synthetic urine and authentic patient urine samples
β-glucuronidase is an enzyme commonly utilized in clinical and forensic labs to convertdrug metabolites present in biological fluids into their parent compounds for betterdetection and quantification. In the case of urine drug testing, urine samples are highlyheterogeneous and range from pH 4.6 to pH 8.0 (1), though pH may be outside this
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