[2,15–17] Because the nature of DMAA as a natural supplement is in question, in 2012 the US Food and Drug Administration (FDA) sent letters to supplement companies requesting detailed safety information.[18] Subsequently, supplement manufacturers have begun to change their formulations, advertising, and product labels. However, products containing DMAA are still commonly available in stores and over the Internet, and more recently, DMAA has been found as a component of abused drug formulations, in particular ‘legal high’ designer drug mixtures in the USA.[2,19] Because DMAA was identified as present in multiple non-traumatic deaths in the US military,[15] in early 2012 products containing DMAA were removed from military bases and their use was prohibited pending further testing on their health effects. In early 2013, the FDA issued a warning, listing the substance as dangerous and it is now illegal for military personnel to consume any product that contains DMAA.[20–22] Regardless, due to the association of DMAA with military fatalities, sports doping, and abused drug mixtures, testing by such agencies may need to specifically include it in routine screenings. A common sequence in routine screenings is to perform a rapid, preliminary assay, such as an immunoassay, followed by confirmatory mass spectrometry methods. For example, it is documented that branches of the US military employ three tests to report a positive result in urine screening, including two .....