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无锡微色谱生物科技有限公司

见微知著，样品分析，如虎添翼

A Sensitive Liquid Chromatography–Tandem Mass Spectrometry Method for the Measurement of 11β-MNT and 11β-MNTDC in Human Serum
来源: | 作者:Feng Bai, Vignesh Iyer, Ronald Swerdloff, and Christina Wang | 发布时间: 1554天前 | 2933 次浏览 | 分享到:

A Sensitive Liquid Chromatography–Tandem Mass Spectrometry Method for the Measurement of 11β-MNT and 11β-MNTDC in Human Serum

-Summary of MSACL 2017 Poster Presentation by: Feng Bai, Vignesh Iyer, Ronald Swerdloff, and Christina Wang; Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center

HybridSPE® Extraction

11-beta-methyl-19-nortestosterone (11β-MNT) is the active androgen derived from 11-beta-methyl nortestosterone dodecylcarbonate (11β-MNTDC). 11β-MNT is being developed as a candidate drug of male hormonal contraception. 11β-MNT like dimethandronlone (DMA) binds to both the androgen and progesterone receptor and may provide the suppression of spermatogenesis with one compound instead of two agents, testosterone and a progestin. The objective of this study was to develop and validate a highly sensitive LC-MS/MS method for the simultaneous detection of serum 11β-MNT and 11β-MNTDC levels for research and clinical purposes in men.

Protein Precipitation

Serum Sample + I.S. + ACN

Vortex & Centrifuge (for increased efficiency use Tip-on-Tip)

Transfer supernatant

CONDITION

Aspirate and Dispense HybridSPE- XTR Tips with 500 μL acetonitrile

Extraction

Aspirate and dispense the supernatant 2x with HybridSPE- XTR Tips to bind interferents to sorbent

Transfer remaining clean sample to a new glass vial

Prep for injection

Dry down the extracted samples

Reconstitute with 40% MeOH in 0.1% formic acid H2O for the analysis