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无锡微色谱生物科技有限公司

见微知著，样品分析，如虎添翼

A Sensitive Liquid Chromatography–Tandem Mass Spectrometry Method for the Measurement of Nestorone in Human Serum
来源:DPX | 作者:Feng Bai, Vignesh Iyer, Ronald Swerdloff, and Christina Wang | 发布时间: 1920天前 | 3131 次浏览 | 分享到:

A Sensitive Liquid Chromatography–Tandem Mass Spectrometry Method for the Measurement of Nestorone in Human Serum

-Summary of MSACL 2017 Poster Presentation by: Feng Bai, Vignesh Iyer, Ronald Swerdloff, and Christina Wang; Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center


HybridSPE® Extraction

  • Nestorone® (NES) is a synthetic progestin being developed by Population Council and NICHD for female and male contraception.
  • NES does not bind to the androgen or the estrogen receptor and may have less adverse effects than androgenic progestin commonly used for female contraception.
  • NES was previously measured in serum by radioimmunoassay that has non specific interference leading to detectable NES levels in men when no NES was administered.
  • This objective of this study was to develop and validate a highly sensitive LC MS/MS method for the detection of serum Nestorone levels for research and clinical purposes in men.


Protein Precipitation       Serum Sample + I.S. + ACN with 1% Formic Acid
Vortex & Centrifuge (for increased efficiency use Tip-on-Tip)
Transfer supernatant
Condition Aspirate and dispense HybridSPE- XTR Tips with 1000 μL acetonitrile
Extraction Aspirate and dispense the supernatant 2x with HybridSPE- XTR Tips to bind interferents to sorbent
Transfer remaining clean sample to a new glass vial
Prep for injection Dry down the extracted samples
Reconstitute with 40% MeOH in 0.1% formic acid H2O for the analysis

You can automate this method with Tip-on-Tip for protein precipitation and a HybridSPE XTR tip format for an automated liquid handler.