Overview:
Marijuana is a commonly used illicit drug and the active constituent,tetrahydrocannabinol (THC), is rapidly oxidized and metabolized into
11-nor-∆9-tetrahydrocannabinol-9-carboxylic acid (THCA), which is conjugatedto form glucuronides in biological systems. In order to accurately quantify thesemetabolites by LC-MS/MS from urine samples, the glucuronide conjugates arehydrolyzed. Te use of IMCSzyme®for hydrolysis of THCA-glucuronide againstcrude enzyme was investigated.