PO0013 Enzyme Activities (Fishman units) Correlate Poorly with Hydrolysis Efficiencies
来源:IMCS
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作者:Cathleen Melendez*, Margarita Marinova, P. Nikki Sitasuwan, L. Andrew Lee
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发布时间: 1714天前
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2015 次浏览
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•Activity of five differentβ-glucuronidases was tested via a phenolphthalein-glucuronide chromogenic assay.
•Hydrolysis efficiency of ten drugs was monitored for all fiveβ-glucuronidases at fivetime points.
•Activity level measured with phenolphthalein-glucuronide did not correlate well withdrug hydrolysis efficiency.
•Hydrolysis efficiency of ten drugs was monitored for all fiveβ-glucuronidases at fivetime points.
•Activity level measured with phenolphthalein-glucuronide did not correlate well withdrug hydrolysis efficiency.
β-glucuronidase is an enzyme commonly utilized in clinical and forensic labs toconvert drug metabolites present in biological fluids into their parent compoundsfor better detection and quantification. Since Fishman et al. introduced a quickand simple way to determineβ-glucuronidase activity in the 1940s, the
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