PO0025 Modular Automated Sample Extractions using IMCStips™on Hamilton® Microlab STAR™for High Throughput Protein Purifications
来源:IMCS
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作者:P. Nikki Sitasuwan1, Todd Mullis1, Caleb Schlacter1, Claire Collins1, Casey Snodgrass2, L. Andrew Lee1*
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发布时间: 1714天前
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1788 次浏览
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INTRODUCTION
Automating complex, multi-step methods requires personnel with laboratory science,robotics programming skills, and design of experiment expertise. They must invest a significant amount of time outlining the workflow and transferring methods for robotic automation and obtaining such resources can be tasking. Here, we automated thecomplex phosphopeptide enrichment methods starting from crude cell lysate to liquidchromatography-mass spectrometry (LC-MS) ready processed samples as an example.Protein therapeutics continue to expand as a major class of new drug products. Research and development into proteins require a purification processes using a range of resins. Integrated workflows with IMCStips demonstrate purification of different proteins using different INtip™ chemistries and the flexibility of the Hamilton MicrolabSTAR to achieve high throughput workflows with minimal human intervention.
•Automated protein A (MabSelect SuRe) INtip purification and buffer exchange in 2 hours.
•Peptide mapping using denaturation, reduction, alkylation and trypsin digestion.
•Examples of modular programs to run for small proof of concept experiments 8-24 samples to larger scale sample processing 96 sample batches.
•Modular pre-templated programs for protein digestion, peptide desalting and phosphopeptide enrichment run in single run or in three separate stages
阅读原文
Automating complex, multi-step methods requires personnel with laboratory science,robotics programming skills, and design of experiment expertise. They must invest a significant amount of time outlining the workflow and transferring methods for robotic automation and obtaining such resources can be tasking. Here, we automated thecomplex phosphopeptide enrichment methods starting from crude cell lysate to liquidchromatography-mass spectrometry (LC-MS) ready processed samples as an example.Protein therapeutics continue to expand as a major class of new drug products. Research and development into proteins require a purification processes using a range of resins. Integrated workflows with IMCStips demonstrate purification of different proteins using different INtip™ chemistries and the flexibility of the Hamilton MicrolabSTAR to achieve high throughput workflows with minimal human intervention.
•Automated protein A (MabSelect SuRe) INtip purification and buffer exchange in 2 hours.
•Peptide mapping using denaturation, reduction, alkylation and trypsin digestion.
•Examples of modular programs to run for small proof of concept experiments 8-24 samples to larger scale sample processing 96 sample batches.
•Modular pre-templated programs for protein digestion, peptide desalting and phosphopeptide enrichment run in single run or in three separate stages
阅读原文
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