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PO0019 Increased Throughput of INtip Affinity Purification for Larger Sample Volume
来源:IMCS | 作者:P. Nikki Sitasuwan1*, Huey J. Nguyen1, Todd Mullis2, Chris Kemp3, Casey Snodgrass4, L. Andrew Lee1* | 发布时间: 1714天前 | 1832 次浏览 | 分享到:
Dispersive pipette extraction is a patented INtip™ micro-extraction platform that leverages turbulent mixing of the resinwithin the pipette tip to increase interaction time between resin and sample. Coupling this core technology to the highlyflexible, automated liquid handling system – Hamilton® Microlab STAR™ workstation – enables rapid INtip purification ofantibodies, recombinant proteins and various other recombinant proteins in a consistent manner. Here, we address one ofthe major challenges in high throughput monoclonal antibody purification, which has been processing sample volumes over3 mL using INtip micro-purification technologies. The workflow is outlined inFigure 1.
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