Dispersive pipette extraction is a patented micro-extraction platform that leverages turbulent mixing within the pipette tip toincrease interaction time between the loose resin and the liquid sample. Coupling this core technology to the highly flexible,automated liquid handling system – Hamilton Robotics – enables rapid enrichment of antibodies, recombinant proteins andvarious other tagged proteins in a consistent manner. Unlike the traditional chromatography columns which rely on fixedbeds, dispersive pipettes contain loose resin with a large void between the two porous filters. Here, we demonstrate theflexibility of the micro-extraction platform on Hamilton STAR to purify milligram scale antibodies from 1 mL aliquots, as wellas purification of his-tagged recombinant proteins from micro-cultures in 96 well format. The “ready to go” workflows provideeluted proteins that are processed through high throughput buffer exchange on the liquid handling platform to ensure a fullyautomated laboratory process starting from crude samples to purified and buffered samples.