Pretreatment | Homogenize Add ACN vortex/centrifuge Evaporate supernatant Reconstitute |
Condition | Aspirate and dispense 100% MeOH Aspirate and dispense 100% H2O |
Bind | Aspirate and dispense sample solution 4x |
Wash | Aspirate and dispense 100% H2O Aspirate and dispense 1% FA H2O Aspirate and dispense 100% MeOH |
Elute | Aspirate and dispense 5% NH4OH MeOH |
XTR Tips for Hamilton
XTR tips with Strong Cation Exchange (SCX) sorbent were used in this method.
Tip Format Options:
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