传统尿液药物分析是用鲍鱼提取的β-葡萄糖醛酸酶进行的,然而,动物提取酶杂质含量高,酶活相对较低,对后期纯化要求较高,增加了实验的繁琐性。2014年,某疼痛药物监控实验室在美国临床化学(AACC)年会上提出:应用IMCSzyme®——一种新型重组的β-葡萄糖醛酸酶可更大的提高尿液中药物检测与分析效率。这项研究成果无疑为医学实验室的药物检测奠定了夯实的基础。
室温下,0 min时,确诊患者尿样经重组β-葡萄糖醛酸酶水解产物的回收率。
* % 相对于鲍鱼提取酶水解浓度
参考文献
1. Rapid Enzyme Hydrolyis Using a Novel Recombinant b-glucuronidase in Benzodiazepine Urine Analysis. Poster Presentation by Ayodel A. Morris, Scott
A. Chester, Erin C. Strickland, and Gregory L. McIntire in Spring 2014, CAC Seminar
2. IMCSzyme® Package Insert, Catalog No. 04-E1F-050. Integrated Micro-Chromatography Systems, Columbia, SC, 2014.
This information was summarized by IMCS from “Rapid Enzyme Hydrolyis Using a novel recombinant b-glucuronidase in Benzodiazepine Urine Analysis” by Ayodele A.
Morris, Scot A. Cester, Erin C. Strickland and Gregory L. McIntire in the Journal of Analytical Toxicology 2014; 38:6 10 - 614
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