JA0010 Determination of Drugs in Brain Samples using Disposal Pipette Extraction
来源:Toxichem Krimtech
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作者:Verena Poetsch , Kurt Jiran , Karin Simon , Thomas Stimpfl
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发布时间: 2156天前
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1910 次浏览
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Aim: The aim of this study was to evaluate a new technique for the manual extraction of different
drugs from postmortem brain samples.
Methods: Homogenized brain tissue from a pig was spiked with a mixture of analytes.
For sample pre-treatment, protein precipitation with acetonitrile was compared to direct dilution
with phosphate buffer.
Disposal pipette extraction tips were loaded by hand with a syringe device. The extraction
method was optimized and the drugs were detected by GC-MS-SIM after silylation.
Results: Most of the drugs analysed showed reproducible recovery rates. Recoveries were
dependent on sample pre-treatment. It could be shown that avoiding protein precipitation resulted
in more reliable results.
Discussion: Analytes with different physico-chemical properties were extracted from a complex
biological matrix by manual Disposable Pipette Extraction. The homogenous mixture of
the sorbent with the sample solution was essential for reliable results.
Conclusion: The developed method can be used for the manual extraction of complex postmortem
brain samples in cases where no (automated) extraction devices for SPE are available.
drugs from postmortem brain samples.
Methods: Homogenized brain tissue from a pig was spiked with a mixture of analytes.
For sample pre-treatment, protein precipitation with acetonitrile was compared to direct dilution
with phosphate buffer.
Disposal pipette extraction tips were loaded by hand with a syringe device. The extraction
method was optimized and the drugs were detected by GC-MS-SIM after silylation.
Results: Most of the drugs analysed showed reproducible recovery rates. Recoveries were
dependent on sample pre-treatment. It could be shown that avoiding protein precipitation resulted
in more reliable results.
Discussion: Analytes with different physico-chemical properties were extracted from a complex
biological matrix by manual Disposable Pipette Extraction. The homogenous mixture of
the sorbent with the sample solution was essential for reliable results.
Conclusion: The developed method can be used for the manual extraction of complex postmortem
brain samples in cases where no (automated) extraction devices for SPE are available.
1. Introduction
The analysis of drugs of abuse in biological specimens requires complex techniques in order to eliminate interferences of endogenous compounds and to obtain reproducible and efficient results. In some toxicological laboratories, tissue extractions are not performed on a routine basis, and the acquisition of an (automated) extraction device for solid-phase extraction (SPE) is therefore not useful. Disposal Pipette Extraction (DPX), which is a novel SPE method using a loosely packed sorbent in a disposal pipette tip, can be performed manually using a syringe device for loading the pipette tip.
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The analysis of drugs of abuse in biological specimens requires complex techniques in order to eliminate interferences of endogenous compounds and to obtain reproducible and efficient results. In some toxicological laboratories, tissue extractions are not performed on a routine basis, and the acquisition of an (automated) extraction device for solid-phase extraction (SPE) is therefore not useful. Disposal Pipette Extraction (DPX), which is a novel SPE method using a loosely packed sorbent in a disposal pipette tip, can be performed manually using a syringe device for loading the pipette tip.
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