注册后可尊享会员价!

电子邮箱

密码

安全问题

注册 忘记密码?
无锡微色谱生物科技有限公司
MicroSep Biotechnology Co. LTD.
  您的当前的位置:
   技术文献

   PAPERS

JA0050 New Approach to Multiresidue Pesticide Determination in Foods with High Fat Content Using Disposable Pipette Extraction (DPX) and Gas Chromatography-Mass spectrometry (GC-MS)
来源:Journal Of Agricultural and Food Chemistry | 作者:HONGXIA GUAN | 发布时间: 87天前 | 215 次浏览 | 分享到:
Organochlorine and organophosphate pesticides in corn muffin mix and cocoa beans were analyzed using disposable pipette extraction (DPX) for rapid cleanup followed by gas chromatography-mass spectrometry (GC-MS). The DPX method in this study used weak anion exchange (WAX) mechanisms to remove the major sample matrix interferences, fatty acids, from the chromatographic analyses. The limits of detection (LOD) were determined to be
1 Introduction

Pesticides continue to contribute significantly to controlling and destroying various types of agricultural pests and thereby improving food production throughout the world. However, uncontrolled pesticide use has led to the deaths of animals and humans (1). The publication of Rachel Carson’s book Silent Spring (2) is often acknowledged for increasing awareness of the potential health hazards posed by pesticides. Routine and comprehensive testing of multiresidue pesticides in food is important for regulatory agencies to ensure that concentrations of toxic pesticides are below tolerance levels. Analysis of target pesticides at low concentrations, particularly in grain products and other food products of high fat content, requires cleanup or elimination of matrix interferences prior to chromatographic and mass spectrometric analysis. Although liquid-liquid extraction and gel permeation chromatography (GPC) have been commonly employed for cleanup with multiresidue pesticide analysis (3, 4), solid-phase extraction (SPE) largely replaced these traditional methods due to its selectivity and elimination of large volumes of organic solvents (5-9). The presence of fatty acids produces severe interferences with gas chromatography of pesticides, and their removal is necessary prior to analysis. In recent years, there has been a great amount of interest in using SPE to remove sample matrix components from extracts, with most research focused on reducing or eliminating fatty acids (3-5, 10, 11). Florisil, strong anion-exchange sorbents (SAX) (5, 12), and weak anion-exchange sorbents including primary-secondary amine (PSA) (13, 14), aminopropyl (-NH2) (15, 16), and diethylaminopropyl (DEA) (17) have been investigated for removal of fatty acids naturally occurring in food samples. Of the sorbents involved in previous studies, Florisil was found to be unsuitable for multiresidue pesticide analysis because it strongly adsorbs polar pesticides, such as organophosphate pesticides (18). SAX was also reported to have little effect on the removal of fatty acids (5, 19). The aminopropyl and PSA sorbents were found to provide the most effective cleanup (5), with PSA being more efficient due to its higher capacity compared to aminopropyl (14, 20)

.....



阅读全文
上一篇: JA0049 PES......
下一篇: JA0051 The......
              我要留言
              在线专家为您解答