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无锡微色谱生物科技有限公司

见微知著,样品分析,如虎添翼

PO0027 How DPX and Micro Luke Works at SCDA to Screen Produce for Pesticide Residue
来源:SCDA | 作者:Sherry T. Garris and Chanin Craft | 发布时间: 2163天前 | 1812 次浏览 | 分享到:
A Modified Micro Luke Method and Disposable Pipette Extraction (DPX) method is described that is used for the extraction of polar and nonpolar pesticides, respectively. This method takes advantage of the use of selective detectors for gas chromatography (GC) such as a nitrogen-phosphorous detector (NPD) and electron capture detector (ECD). Also, this method can be used with mass spectrometry (MS) for GC (or high performance liquid chromatography (HPLC) following solvent exchange).

The extractions are first processed using acetone, and one portion of the acetone solution undergoes a rapid liquid-liquid extraction for the polar pesticides, such as organophospates and organo-nitrogen pesticides.  The 2nd portion of acetone is processed using reversed phase mechanisms with DPX tips. The time-consuming column chromatography in the Luke Method for GC-ECD analysis of organochlorine pesticides is not required. The DPX extracts result in chromatograms that have low background interferences for a wide variety of fruit and vegetables. In fact, the ECD chromatograms obtained by using DPX are comparable to those obtained by the Luke Method.




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