Accurate and robust quantification of insulin-like growth factor 1 (IGF-1) from serum using mass spectrometry (MS) is challenging due to its high complexity and dynamic range. Low cost,high throughput enrichment method for such low abundant proteins from the serum is required to enrich target proteins prior to MS analysis. We report a high-throughput sample reparation method using reverse phase micro-solid phase extraction enrichment on Microlab® NIMBUS® robotic liquid handling system from Hamilton Robotics and demonstrated over 71% recovery from serum for 96 samples in less than 30 minutes. IGF-1 levels in serum correlates well to other published methods that involve longer processing times.