PO0006 Automated Immunoaffinity Enrichment for Phosphotyrosine Peptides Using Protein A andStreptavidin IMCStips
来源:IMCS
|
作者:Sunil Hwang*1,Todd Mullis2, Michael Walla2, Matthew Manter1, Jingyun Lee3, Christina M Furdui3, L. Andrew Lee1
|
发布时间: 2168天前
|
2685 次浏览
|
分享到:
•Established automated Protein A and Streptavidin immunoaffinity enrichment protocol for high-
throughput sample preparation
Identified over 1000 phosphotyrosine peptides using anti-phosphotyrosine antibodies and Protein A /Streptavidin IMCStips
•Achieved over 94% specificity of phosphotyrosine peptide enrichment using Hamilton® Microlab STAR workstation and IMCStips
throughput sample preparation
Identified over 1000 phosphotyrosine peptides using anti-phosphotyrosine antibodies and Protein A /Streptavidin IMCStips
•Achieved over 94% specificity of phosphotyrosine peptide enrichment using Hamilton® Microlab STAR workstation and IMCStips
Signal transduction of protein phosphorylation is a key mechanism for survival, maintenance, and deathof eukaryotic cells (1-4). Te global enrichment of phosphoserine and phosphothreonine peptides hasbeen successfully optimized using immobilized metal affinity chromatography (IMAC) and titaniumdioxide (TiO2) using IMCStips with an automated liquid handling system. However, enrichment ofphosphotyrosine (p-Tyr) peptides are less efficient with these methods due to the low stoichiometry of p-Tyr peptides to global phosphorylation event (5-11). Here, we introduce anti-phosphotyrosineantibody enrichment using a dispersive pipette extraction technology in IMCStips. Te method uses anautomatic liquid handling system to enrich p-Tyr peptides for high-throughput and reproducible samplepreparation.
阅读全文
阅读全文
-
2021-09-20
简要介绍 dSPE 在全自动移液工作站上应用实现全自动抗体纯化的原理。
比较了三种 Protein A 纯化 HIgG 的效率及纯化流程优化方法。 -
使用 IMCSTips 实验全自动纯化（一步）方法及数据分析
2021-09-20
1. IMCStips 应用原理展示
2. His-tagged 蛋白纯化流程优化及数据分析 -
2021-09-20
IMCStips 亲和层析柱
● 一次层析即可快速产生高纯重组蛋白
● 一致性好、回收率高，个样品的差异不到 10%
● 可结合各种复杂方法的模板化工作流
● 与 Hamilton 移液工作站结合，可在 30 分钟内处理 96 个样品
● 可定制的工作流程 -
2020-02-08
BenchWaver™ 把传统的平台摇摆运动与轨道振动器的圆周运动结合起来，这种最优组合能够产生最适合分子生物学应用的三维运动。
-
2020-02-08
强力混匀，适于细胞裂解、研磨或均质
与通用的组织研磨机更快速、更高效
采用密封2.0mL管，消除交叉感染
中通量，可同时处理3~6个样品
无锡微色谱生物科技有限公司
WuXi MicroSep Biological Science Co,. Ltd.
地 址：江苏省江阴市月城镇北环路 19 号
电 话：17715681752
工作时间：周一 ~ 周五 9:00 ~ 16:00