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无锡微色谱生物科技有限公司

见微知著,样品分析,如虎添翼

名称描述内容

REVERSE PHASE

OVERVIEW


We offer five desalting resins for small peptide to large biomolecule desalting and enrichment. These resins are often used to bind peptides, nucleotides, and proteins in high-aqueous solutions containing ion suppressing salts and buffers. After binding peptides to the reverse phase resin, the salts and buffers are washed off and the peptides are eluted using a high organic solution, such as methanol or acetonitrile.


BENEFITS


  • Consistent, high recoveries resulting in less than 10 percent variance across 96 samples
  • Fully automated process with little or no manual intervention
  • Templated workflows that combine complex methods
  • Delivered with Hamilton Microlab STAR ™ pre-programmed software scripts with an intuitive user interface to control sample volumes, aspirate/dispense cycles, and wash steps

            Before                              After

Before and after desalting of peptides (from tryptic digested proteins) on MALDI-TOF by IMCStips (a) and (b) were before and after extracted of tryptic Human albumin by IMCStips, (c) and (d) were before and after extracted of tryptic Human α-acid-glycoprotein by IMCStips, (e) and (f) were before and after extracted of tryptic Human pre-albumin by IMCStips.