无锡微色谱生物科技有限公司
见微知著，样品分析，如虎添翼
产品
文章
名称描述内容
PHOSPHOPEPTIDE
OVERVIEW BENEFITS
- A range of ion exchange resins with varying porosities and different degrees of charges
- Consistent, high recoveries resulting in less than 10 percent variance across 96 samples
- Fully automated process with little or no manual intervention
- Templated workflows that combine complex methods
- Delivered with Hamilton Microlab STAR ™ pre-programmed software scripts with an intuitive user interface to control sample volumes, aspirate/dispense cycles, and wash steps
The IMCStips® Phosphopeptide product line is designed for automated high throughput with a customizable screening workflow. We offer automated scripts that allow the user to screen different wash and elution buffers and a number of different resins in a single experiment.
Using metal oxides, phosphopeptides from cell lysates are enriched. The global enrichment of these phosphoserine and phosphothreonine peptides is optimized using zirconium dioxide and PolyTi™ resins in IMCStips.
The different distribution of singly-, doubly- and triply- phosphorylated peptides enriched using the different resins.
Venn diagrams of phosphopeptide overlap from duplicate sample enrichments. The duplicate sample preparations for PolyTi (A), ZrO 2(B) and the combination of both resins in a single pipette tip (C) show an average of 76, 78, and 71 percent overlap, respectively. In comparison, the two different resins PolyTi and ZrO 2show 48 percent of overlap (D). This reduction in overlap of phosphopeptide coverage is expected for these two resin types, as the distribution of the singly- and doubly-phosphorylated peptides were also varied for the two resin types.
