无锡微色谱生物科技有限公司
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AFFINITY
OVERVIEW
Affinity applications are used for IgG isolation, recombinant protein purification, anti-drug antibody assay, therapeutic drug monitoring, and more. Because we understand that the needs of a laboratory are often individualized and specific, IMCStips® are available in a variety of resins designed to optimize multiple testing processes. The combination of our patented dispersive technology and customized resin options facilitate consistent, high recoveries of significant analytes.
BENEFITS
- Rapidly yields small but valuable quantities of pure recombinant protein in a single chromatography step
- Consistent, high recoveries resulting in less than 10 percent variance across 96 samples
- Process 96 samples in minutes
- Templated workflows that combine complex methods
- Delivered with Hamilton Microlab STAR™ pre-programmed software scripts with an intuitive user interface to control sample volumes, aspirate/dispense cycles, and wash steps
HIGH PERFORMANCE AND CONSISTENCY
1 mL IMCStips with 50 µL of Protein A resin was used at increasing aspirate/dispense cycles to improve the final yield of IgG as indicated by protein quantification (UV-Vis Abs @ 280 nm) and also by the depletion of the heavy and light chains in the flow through media. The eluants are after 20x aspirate/dispense cycles followed by a final elution volume of 400 mL in NaOAc pH 3.5. The sample was neutralized with 1/10 volume of 1 M Tris pH 8. Each cycle is a duplicate run with the exception of the final 20x flow through and eluants which are in triplicates.
1 mL IMCStips with 50 µL of Protein A resin was used for the enrichment of mAb from cell media. It was tested over three days using a different number of tips and two different aspirate/dispense cycles.